Treatment with (R)-α-methylhistamine or IL4 stimulates mucin production and decreases Helicobacter pylori density in the murine stomach

A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.

A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.

A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.

A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.