Treatment with (R)-α-methylhistamine or IL4 stimulates mucin production and decreases Helicobacter pylori density in the murine stomach
Citation and access
Citation and access
Creator/Principal investigator(s):
- Macarena P. Quintana-Hayashi - University of Gothenburg
Research principal:
Data contains personal data:
No
Citation:
Data access level:
Language:
Method and outcome
Method and outcome
Population:
Mice were divided into different groups: non-infected vehicle-treated control, H. pylori vehicle-treated, H. pylori-infected RaMH-treated and H. pylori-infected Il4-treated.
Time method:
Study design:
- Preclinical study
Description of study design:
This study aimed to restore mucin production in the male C57BL/6 mouse H. pylori (SS1) infection model and evaluate its effects on H. pylori density. Mice infected with SS1 were treated with (R)-α-methylhistamine (RαMH) or interleukin-4 (IL4).
Sampling procedure:
Description of sampling:
A total of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO and diluted in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.
Time period(s) investigated:
Variables:
26
Number of individuals/objects:
86
Data collections - 4 collections
Data collections - 4 collections
Data collection - Measurements and tests
Data collection - Measurements and tests
Mode of collection:
Measurements and tests
Description of the mode of collection:
A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.
Time period(s) for data collection:
2021-04-22 - 2021-05-06
Source of the data:
- Biological samples
Data collection - Measurements and tests
Data collection - Measurements and tests
Mode of collection:
Measurements and tests
Description of the mode of collection:
A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.
Time period(s) for data collection:
2021-11-17 - 2021-12-02
Source of the data:
- Biological samples
Data collection
Data collection
Description of the mode of collection:
A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.
Time period(s) for data collection:
2022-03-24 - 2022-04-09
Source of the data:
- Biological samples
Data collection
Data collection
Description of the mode of collection:
A total volume of 300 µl per mouse was prepared by dissolving 2.6 mg of GalNAz in 50 µl DMSO followed by dilution in PBS. The GalNAz injections were given intraperitoneally at 14 days post-infection, and mice were sacrificed 2 h after the injection by cervical dislocation under anesthesia with isoflurane. Whole blood samples from each mouse were collected upon sacrifice. Then, the stomachs were harvested and opened along the greater curvature followed by a gentle wash in sterile PBS to remove chyme. A stripe of the lesser curvature of the stomach containing forestomach, corpus, and antrum was fixed in buffered formaldehyde 4% aqueous solution for 24 h. The fixed tissue was paraffin-embedded and histological slides were prepared with 4 µm thick tissue ribbons. Additionally, two pieces of the corpus (approximately 4 x 4 mm) were collected. One was placed in RNA-later, and stored at 4°C, the other one was immediately frozen in dry ice. Both samples were later stored at -80°C until use.
Time period(s) for data collection:
2022-09-07 - 2022-09-22
Source of the data:
- Biological samples
Administrative information
Administrative information
Responsible department/unit:
Department of Medical Biochemistry and Cell biology
Ethics Review:
Swedish Ethical Review Authority - 52-2021
The Göteborgs Djurförsöksetiska Nämnd (Ethic No. 52-2021) approved all experimental procedures based on the regulation from Djurskyddsförordningen DFS 2004:4.
Topic and keywords
Topic and keywords
CESSDA Topic Classification:
Standard för svensk indelning av forskningsämnen 2025:
Publications
Publications
Citation:
Citation:
Citation:
Metadata
Metadata
