Acute high-dose irradiation disrupts cell adhesion and Silk-Ovarioid formation in human primary ovarian cells
Citation and access
Citation and access
Data access level:
Creator/Principal investigator(s):
Research principal:
Data contains personal data:
Yes
Type of personal data:
Transcriptomic data
Code key exists:
Yes
Sensitive personal data:
Yes
Citation:
Language:
Method and outcome
Method and outcome
Population:
The samples were obtained from patients undergoing elective abdominal surgery recruited from Karolinska University Hospital, Huddinge, Sweden.
Study design:
- Experimental study
Sampling procedure:
Description of sampling:
The samples were collected from random patients undergoing elective abdominal surgery and consent to give ovarian tissues to research.
Number of individuals/objects:
5
Data format/data structure:
Samples/material - Collected from scientific collection/biobank
Samples/material - Collected from scientific collection/biobank
Name:
Type(s) of sample:
Geographic coverage
Geographic coverage
Geographic location:
Administrative information
Administrative information
Responsible department/unit:
Department of Women's and Children's Health [K6]
Ethics Review 1:
Swedish Ethical Review Authority - 2015-798/31
Ethics Review 2:
Swedish Ethical Review Authority - 2024-08606-01
Funding
Funding
Funding agency:
- Swedish Research Council
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Award number:
2024-02530_VR
Award title:
Common factors contributing to poor reproductive outcome from failed implantation to pregnancy loss
Funding information:
Motherhood holds a profound significance for many women, yet one in six couples encounters infertility. In vitro fertilization (IVF), the most common treatment, yields modest pregnancy rate. Moreover, 15% of all pregnancies are spontaneously miscarried. Embryo implantation and pregnancy progression rely on communication between genetically normal, euploid embryo and uterus, complemented by favorable microbiota. Even following the transfer of the tested euploid embryo, a maximum implantation rate of 50% is achieved, causing the need for multiple transfers. The fact that failed implantation and pregnancy loss are common, underscores the importance of hostile endometrial environment, impaired embryo-maternal communication, and dysbiotic uterine microbiota in poor fertility. PODIUM project adopts a holistic approach to unravel the interplay between endometrial, embryonic, and host microbiota factors underlying the etiology of implantation failure and pregnancy loss. We adopt systems biology approach, based on our expertise in conducting integrated multiomic and microbiota profiling of endometrium. This approach will be augmented by using our in vitro implantation models, including embryo-like blastoids and endometrial organoids, to delve into implantation mechanisms. Thus, PODIUM contributes to the clinical field, which is often neglected, and paves the way for enhanced diagnostic abilities and the development of personalized treatments for female poor reproductive outcome.
