Rodrigues et al. The genetic basis and adult reproductive consequences of developmental thermal plasticity

Summary: This data was used to assess how sublethal heat stress during development impacted subsequent multiple reproductive traits in both sexes and their plasticity and to identify candidate genes of reproductive traits sensitive to thermal stress. Using a subset of isogenic lines of the Drosophila Genetic Reference Panel (DGRP) reared at standardized densities in three temperatures (25°C, 27°C, 29°C), we quantified phenotypic variation in mating propensity, fertility, productivity and sex-ratio and obtained their respective thermal reaction norms when applicable. Files available: We provide 2 .txt files (MALE_phenotypicdata; FEMALE_phenotypicdata) and a .xls file (TableS4_genomic_analyses_results_productivity). MALE_phenotypicdata: This file contains the raw data obtained for male DGRP under one of three constant temperatures. Table headers are explained below: Block – week in which the data were collected (1 or 2). LineDGRP - line identity. Line_Status - classification based on the male reproductive performance across three temperatures tested in Zwoinska et al. (2020)*. (High, lines whose fertility did not decline as temperature increased; Low, lines whose fertility declined substantially as temperature increased). Temperature - mean developmental temperature experienced (25°C, 27°C or 29°C). Vial – vial where each pair was kept. Mating – mating propensity (1, if mating observed; 0, if no mating observed) Larvae – fertility (1, if larvae present; 0, if no larvae present) Productivity (1,2 and 3) – number of adult offspring counted on the first, second and third day of offspring counting. Daughters (1, 2 and 3) – number of adult females counted on the first, second and third day of offspring counting. Sons (1, 2 and 3) – number of adult males counted on the first, second and third day of offspring counting. daughtersTotal – Total number of adult females counted. sonsTotal– Total number of adult males counted. productivityTotal– Total number of adult offspring counted. FEMALE_phenotypicdata: This file contains the raw data obtained for female DGRP under one of three constant temperatures. Table headers are explained below: LineDGRP - line identity. Line_Status - classification based on the male reproductive performance across three temperatures tested in Zwoinska et al. (2020)*. (High, lines whose fertility did not decline as temperature increased; Low, lines whose fertility declined substantially as temperature increased). Temperature - mean developmental temperature experienced (25°C, 27°C or 29°C). Vial – vial where each pair was kept. Larvae – fertility (1, if larvae present; 0, if no larvae present) Productivity (1,2 and 3) – number of adult offspring counted on the first, second and third day of offspring counting. Daughters (1, 2 and 3) – number of adult females counted on the first, second and third day of offspring counting. Sons (1, 2 and 3) – number of adult males counted on the first, second and third day of offspring counting. daughtersTotal – Total number of adult females counted. sonsTotal– Total number of adult males counted. productivityTotal– Total number of adult offspring counted. TableS4_genomic_analyses_results_productivity: This table contains several individual sheets, the legends for these sheets are given on the first sheet of the excel and on the files called "Figshare_information_file". *Zwoinska, M. K., Rodrigues, L. R., Slate, J., & Snook, R. R. (2020). Phenotypic responses to and genetic architecture of sterility in response to sub-lethal temperature during development. Frontiers in Genetics, 11, 573. https://doi.org/10.3389/fgene.2020.00573Opens in a new tab