Fig. 5h-l | Mitochondrial protein expression by quantitative immunofluorescence microscopy

This item is part of the Figshare Project: Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution (https://su.figshare.com/projects/Early_mitochondrial_dysfunction_revealed_across_FUS-_and_TARDBP-ALS_at_single_cell_resolution/163252Opens in a new tab) From Data Availability Statement for the forthcoming paper in Nature Communications entitled: Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS "We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study. [Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files." -------------------------------------------- [Item specific description:] Quantitative immunofluorescence microscopy was used to assess the expression of mitochondrial genes MT-CO1, MT-CO2, MT-CO3, and NDUFA12 in ALS motor neuron axons. These files are confocal images of immunofluorescent stainings of mitochondrial proteins in iPSC-derived motor neurons. The file format is .czi, through the ZEN software (Zeiss). Zeiss recommends using ImageJ and the ImageJ-based Fiji software package (https://imagej.net/software/fiji/Opens in a new tab) . Information about the file format CZI can be found here: https://www.zeiss.com/microscopy/en/products/software/zeiss-zen/czi-image-file-format.htmlOpens in a new tab