LysM phylogeny including Datisca glomerata and Ceanothus thyrsiflorus
https://doi.org/10.17045/STHLMUNI.6384200
Phylogeny of LysM paralogs with sequences harvested from newly sequenced transcriptomes from Datisca glomerata and Ceanothus thyrsiflorus plus the following species as references: Arabidopsis thaliana, Cicer arietinum, Cucurbita pepo, Fragaria vesca, Glycine max, Lotus japonicus, Manihot esculenta, Medicago trunculata, Morus notabilis, Oryza sativa, Prunus persica, Ricinus communis, Solanum lycopersicum, Sorghum bicolor, Theobroma cacao, Vigna radiata and Ziziphus jujuba.
Sequences were collected with blastp searches against the RefSeq database subset by the above list of species, using presumed D. glomerata and C. thyrsiflorus orthologs.
Sequences were aligned with Clustal Omega (http://www.clustal.org/omegaOpens in a new tab; Sievers et al. 2014) and reliable alignment positions were selected with the BMGE algorithm (Criscuolo and Gribaldo 2010) using the BLOSUM62 substitution matrix. Sequences that were identical after BMGE, were discarded.
The tree was estimated with RAxML v. 8.2.4 (https://sco.h-its.org/exelixis/web/software/raxml/index.htmlOpens in a new tab; Stamatakis 2014) using the PROTGAMMAAUTO model and automatic bootstopping.
Files provided are:
1. The full alignment after BMGE selection of positions and removal of identical sequences: lysm.refseq_harvest_plus_selected.co.BLOSUM62.bmge.rx.red.phylip
2. The maximum likelihood tree labelled with bootstrap values in:a. newick format: lysm.refseq_harvest_plus_selected.co.BLOSUM62.bmge.rx.red.PROTGAMMAAUTO.raxml.besttree.newickb. Dendroscope (http://dendroscope.orgOpens in a new tab; Huson et al. 2007): lysm.refseq_harvest_plus_selected.co.BLOSUM62.bmge.rx.red.PROTGAMMAAUTO.raxml.bipartitions.nexml
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Opens in a new tabhttps://doi.org/10.17045/STHLMUNI.6384200
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