Hunting Pumpkinseed (Lepomis gibbosus): A eDNA study in the Kungsbacka area

In July 2018, dead Pumpkinseed fish (Lepomis gibbosus) were found in a river near the church in Älvsåker (Kungsbacka municipality, Halland County). The fish most likely originated from a artificial pond, which via a stream is connected to the location where the dead fish was found. A large number of Pumpkinseed fish were discovered in the pond - something the owner of the pond water was unaware of. It was decided that the pond would be emptied (which happened later in the summer) and kept empty until all the fish were dead. The mission of this investigation was to investigate a number of water bodies (rivers and ponds) with eDNA technology to find out if DNA traces of the fish could be detected in the sourroundings of the source pond. To this end samples were to be taken on a total of ten different premises in Kungsbackaån, Lillån and nearby watercourses (Skårsjöån, Rolfsån, and canal between Kungsbackaån and Rolfsån). It is important to mention that the sampling was performed when it was still so dry that there was no water in the furrow from the pond where the species was present in large quantities. The study and itsresults are reported in the attached publication. The dataset includes 5 files, with 3 log-files (analysis log and error-log), plus 2 result files, with documentation.