Data (bilder) för: Nya streckkoder för Svenska kiselalger från sötvatten

Diatoms were isolated from fresh samples taken in coordination with the Swedish national monitoring program in 2019 and 2020. Those samples were taken following the European standard EN 13946:2025 Water quality - Guidance standard for the routine sampling and preparation of benthic diatoms from rivers and lakes. In total, 273 monoclonal cultures were established from single cells using the micropipette method. Cultures were grown in WC (Guillard and Lorenzen 1972), PM (Stosch and Fecher 1979), or L16 (Lindström 1991) medium at room temperature. Photographs were taken from each living culture if possible. The cultures were subsequently harvested and sequenced, and in parallel identified using morphological characters using light microscopy in a first step. Selected cultures were also studied and pictures taken using scanning electronic microscopy. The clones were harvested when the biomass was sufficient for all further project relevant analysis steps, which included DNA barcode sequencing for rbcL and 18SV4.

Kiselalger har provtagits från 44 olika vattendrag i Sverige, geografiskt spridda över landet från norr till söder (58.3475 to 65.4433 WGS84).