Data (images) for: New barcodes of freshwater diatoms from Sweden
Documentation files
Documentation files
Citation and access
Citation and access
Data access level:
Creator/Principal investigator(s):
Research principal:
Principal's reference number:
- SLU.ma.2025.4.2.IÄ-7
Data contains personal data:
No
Citation:
Language:
Method and outcome
Method and outcome
Time period(s) investigated:
Data format/data structure:
Species and taxons:
Samples/material - Collected from scientific collection/biobank
Samples/material - Collected from scientific collection/biobank
Name:
Type(s) of sample:
Data collection - Biological tests
Data collection - Biological tests
Mode of collection:
Biological tests
Description of the mode of collection:
Diatoms were isolated from fresh samples taken in coordination with the Swedish national monitoring program in 2019 and 2020. Those samples were taken following the European standard EN 13946:2025 Water quality - Guidance standard for the routine sampling and preparation of benthic diatoms from rivers and lakes. In total, 273 monoclonal cultures were established from single cells using the micropipette method. Cultures were grown in WC (Guillard and Lorenzen 1972), PM (Stosch and Fecher 1979), or L16 (Lindström 1991) medium at room temperature. Photographs were taken from each living culture if possible. The cultures were subsequently harvested and sequenced, and in parallel identified using morphological characters using light microscopy in a first step. Selected cultures were also studied and pictures taken using scanning electronic microscopy. The clones were harvested when the biomass was sufficient for all further project relevant analysis steps, which included DNA barcode sequencing for rbcL and 18SV4.
Time period(s) for data collection:
2020 - 2021
Data collector:
- Swedish University of Agricultural Sciences
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ROR
Source of the data:
- Research data
- Biological samples
- Other
Geographic coverage
Geographic coverage
Geographic location:
Geographic description:
The diatom material has been sampled from 44 different streams in Sweden, geographically distributed over the country from North to South (58.3475 to 65.4433 WGS84).
Administrative information
Administrative information
Responsible department/unit:
Department of Aquatic Sciences and Assessment
Commissioning organisation:
- Swedish Environmental Protection Agency
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ROR
Registration number at commissioning organization:
- 18/171
Handle:
Funding
Funding
Funding agency:
- Swedish Agency for Marine and Water Management
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ROR
Award number:
18/171
Funding agency:
- Swedish Environmental Protection Agency
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ROR
Award number:
18/171
