Data and code for: Sequence specificity in DNA binding is mainly governed by association

Sequence-specific binding of proteins to DNA is essential for accessing genetic information. Here, we derive a simple equation for target-site recognition, which uncovers a previously unrecognized coupling between the macroscopic association and dissociation rates of the searching protein. Importantly, this relationship makes it possible to recover the relevant microscopic rates from experimentally determined macroscopic ones. This post contains kinetic data for the lac repressor and code to analyse and interpret the data in light of this theory. File list:- data_pbm.zip - Data-set for output generated using a protein binding microarray. - data_tirf.zip - Data-set for output generated using TIRF based single molecule localisation microscopy. - data_cas9.zip - Data-set for cas9 binding collected from literature. - data_spr.zip - Data-set for output generated using surface plasmon resonance based methods. - figure_scripts.zip - Set of scripts used to generate all figures. See README file inside for details.