Microscopy data set of mRNA FISH and Protein gene expression measurements in which repressor binding strength is systematically altered.
https://doi.org/10.17044/SCILIFELAB.26425576
This dataset contains the raw data, analysis and code needed to generate the figures presented in the paper "Anti-correlation of LacI association and dissociation rates observed in living cells" (see Related Material). The raw data consists of microscopy images and qPCR files. The analysis and code consist of data analysis, output from the analysis, its post-processing, simulations, and which figure the data was used in.
Experimental data, and analysis scripts are all organized using unique IDs (UID; seen for example in the filenames of experimental data below).
The README.txt file in the Analysis_of_data folder describes which UIDs for data, analysis, code & figure generation to combine for specific figures.
For the microscopy experiments (has the prefix Microscopy_Data_ in their filenames) there is information on the growth condition and which figure the data was used in. All experiments were performed at 30 degrees Celsius.
Details on the output from microscopy experiments can be found in the file MicroscopyInformation.txt.
Go to data source
https://doi.org/10.17044/SCILIFELAB.26425576
Citation and access
Citation and access
Creator/Principal investigator(s):
Research principal:
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Administrative information
Administrative information
Funding
Funding
Funding agency:
- Swedish Research Council
Opens a new window at ror.org.
ROR
Award number:
2020-05137_VR
Award title:
A double-edged sword? Quantifying tradeoffs in CRISPR immunity
Funding agency:
- European Research Council
Opens a new window at ror.org.
ROR
Award number:
885360
Award title:
Biophysics in gene regulation - A genome wide approach
Funding agency:
- Swedish Research Council
Opens a new window at ror.org.
ROR
Award number:
2016-06213_VR
Award title:
The physics of genetic information processing
Funding agency:
- Swedish Research Council
Opens a new window at ror.org.
ROR
Award number:
2018-03958_VR
Award title:
A genome wide approach to replication initiation
Topic and keywords
Topic and keywords
Swedish Standard Classification of Research Subjects 2025:
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Relations
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Metadata
Metadata
