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Full-length 16S rRNA gene long-read sequencing from dried cervicovaginal fluid

https://doi.org/10.17044/SCILIFELAB.28367732

This dataset refers to the 16S rRNA gene long-read sequencing data described in the article "Full-length multiplexed 16S rRNA gene sequencing works well in self-collected decade old cervicovaginal fluid on paper cards" (unpublished). DNA was extracted from two 3 mm punches of paper cards containing dried cervicovaginal fluids that had been stored for up to eleven years at room temperature. Full-length 16S rRNA gene amplicons (~1.5 kb) were generated using the Kinnex 16S protocol followed by long-read sequencing using the Pacific Biosciences (PacBio) Revio system. The raw fastq files consist of de-multiplexed and circular consensus sequencing reads. A more detailed description of the methods can be found in the paper.

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https://doi.org/10.17044/SCILIFELAB.28367732

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scilifelab
Uppsala University