Hypoxia enhances follicle survival and ameliorates markers of metabolic function in human ovarian cortical tissue culture
https://doi.org/10.48723/hnvh-aq19
The overall aim of this study is to investigate the effects of in vitro culture on human ovarian tissue, and to optimize the culture system to study the reproductive toxicity of chemicals in the laboratory.
The dataset is connected to a study that investigates the impact of in vitro culture on ovarian cortical fragments. In total 5 samples (i.e., 3 samples in the fresh group and 2 samples in the cultured group) collected from 3 patients were sequenced in this study.
Ovarian tissues were dissociated into single-cell suspension using mechanical and enzymatic methods and used for library preparation. Single cell RNAseq libraries were prepared using Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 (10X genomics).
The dataset contains 36 fastq files from paired-end RNA sequencing, compressed with GNUzip (gzip). The dataset also contains one md5sum file in txt format to validate data integrity and a metadata file. The approximate total size of the dataset is 96 GB.
Documentation files
Documentation files
Citation and access
Citation and access
Data access level:
Creator/Principal investigator(s):
- Sevastiani Antonouli – University of Thessaly
- Xinchen Liu – Karolinska Institutet - Institutionen för kvinnors och barns hälsa
- Catarina Arnelo – Karolinska Universitetssjukhuset - Institutionen för gynekologi och reproduktionsmedicin
- Karin Pettersson – Karolinska Universitetssjukhuset - Institutionen för gynekologi och reproduktionsmedicin
Research principal:
Data contains personal data:
Yes
Type of personal data:
Transcriptomic data
Code key exists:
Yes
Sensitive personal data:
Yes
Citation:
Language:
Method and outcome
Method and outcome
Population:
The samples were obtained from patients undergoing elective abdominal surgery recruited from Karolinska University Hospital, Huddinge, Sweden.
Study design:
- Experimentell studie
Sampling procedure:
Description of sampling:
The samples were collected from random patients undergoing elective abdominal surgery and consent to give ovarian tissues to research.
Number of individuals/objects:
3
Data format/data structure:
Samples/material - Collected from scientific collection/biobank
Samples/material - Collected from scientific collection/biobank
Name:
KI biobank, collection "Miljökemikalier och kvinnlig fertilitet"
Type(s) of sample:
Ovarian tissue
Data collection
Data collection
Time period(s) for data collection:
2020 - 2024
Instrument
Instrument
Name:
10x Chromium Controller
Geographic coverage
Geographic coverage
Geographic location:
Administrative information
Administrative information
Responsible department/unit:
Department of Women's and Children's Health [K6]
Ethical Review
Ethical Review
Reviewer:
- Etikprövningsmyndigheten
components.catalogue.resource.content.administrativeInformation.ethicalReview.rorId.srText
ROR
Registration number:
2015-798/31
Reviewer:
- Etikprövningsmyndigheten
components.catalogue.resource.content.administrativeInformation.ethicalReview.rorId.srText
ROR
Registration number:
2021-04563
Topic and keywords
Topic and keywords
Swedish Standard Classification of Research Subjects 2025:
Keywords:
Publications
Publications
Citation:
Li, T., Wehrli, L., Antonouli, S., Panagiotou, E.M., Deligiannis, S.P., Liu, X., Hassan, J., Moussaud-Lamodière, E., Arnelo, C., Pettersson, K., Papaikonomou, K., Salumets, A., Damdimopoulou, P., Nisio, V.D., 2026. Hypoxia enhances follicle survival and ameliorates markers of metabolic function in human ovarian cortical tissue culture. Reproductive BioMedicine Online 105918. https://doi.org/10.1016/j.rbmo.2026.105918
Metadata
Metadata
Version 1
